The left and right margins are a montage of silhouttes of CA1 pyramidal neurons. The neurons were individually filled with Alexa Fluor 594 through a patch pipette. The dye was imaged with a custom galvonometer-based mirror scanning microscope with MP light source (Chameleon, Coherent). As the field of view for the imaging system is ~190um x 140um, several 3-dimensional "stacks" were required to capture the entire structure.
Maximum projections of the image stacks were converted to .tiff format and aligned with Adobe Illustrator 10. The images were then rasterized in Adobe Photoshop at 300 dpi. Using the "magic wand" tool (tolerance=25, no anti-aliasing), the silhouttes were extracted from the backgound. The "paint bucket" tool was then used to fill in the background color seen.
You may freely use these images as long as you attribute authorship to Brad Losavio and Peter Saggau.
higher quality, left (780 kB)
higher quality, no background, left (903 kB)
higher quality, right (790 kB)
higher quality, no background, right (904 kB)
